In situ hybridization histochemistry with synthetic oligonucleotides: Strategies and methods
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چکیده
منابع مشابه
In situ hybridization histochemistry with synthetic oligonucleotides: strategies and methods.
In situ hybridization histochemistry is a useful method for localizing specific mRNA and studying the regulation of gene expression in an anatomical context. Previously, classical recombinant DNA and microbiological techniques have been required to identify and nick-translate the cloned DNAs necessary for in situ hybridization experiments. These requirements can be circumvented by the use of sy...
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The distribution of two different molecules can be analyzed within the same embryo using the procedures described below. The protocol for combined whole mount in situ hybridization and immunocytochemistry allows for simultaneous detection of mRNA and protein. The protocol for combined whole mount in situ hybridization and β-galactosidase staining allows for simultaneous detection of mRNA and tr...
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Vasopressin and oxytocin are nonapeptide hormones that regulate water metabolism and lactation, respectively. To study the regulation of the vasopressin and oxytocin genes at the mRNA level, we constructed a series of synthetic oligonucleotides, from 8 to 15 bases in length, for use in filter-blot hybridization assays (Northern blots) of hypothalamic mRNA levels and for primed synthesis of cDNA...
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1 Notation and Abbreviations 2
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ژورنال
عنوان ژورنال: Peptides
سال: 1985
ISSN: 0196-9781
DOI: 10.1016/0196-9781(85)90138-x